FIGURE

Fig 1

ID

ZDB-FIG-190723-1143

Publication

Murdoch et al., 2019 - Intestinal Serum amyloid A suppresses systemic neutrophil activation and bactericidal activity in response to microbiota colonization

Other Figures

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Fig 1

Saa mediates neutrophil behavior <italic>in vivo</italic>.

(A-B) Imaging and quantification of lyz:EGFP⁺ neutrophils recruited to tail wound margin over 6 hours following caudal fin amputation (dashed red line indicates wound margin; scale bar = 250 μm) (n ≥ 24 larvae / genotype at 6 hour time point). (C) Measurement of lyz:DsRed⁺ neutrophil speed from time-lapse imaging in caudal fin tissue over 6 hour period following amputation (n = 4 larvae / genotype, 87–112 cells tracked / genotype). (D-E) Representative images of 6 dpf Tg(lyz:DsRed) WT and saa^-/- larvae (scale bar = 500 μm). (F) Enumeration of intestine-associated lyz:EGFP⁺ cells in 6 dpf larvae (n = 32–40 larvae / genotype). (G-J) Quantitative analysis of lyz:EGFP⁺ neutrophil behavior from time-lapse imaging of distinct anatomical compartments (intestine and trunk, ROIs in panel D) in 6 dpf larval zebrafish (6 larvae / genotype, ≥ 23 cells analyzed / genotype / tissue). Data analyzed by t-test. For panel B, statistical comparisons were performed within each time point. Data are presented as mean ± SEM. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	saa^rdu60/rdu60; nz117Tg
Condition:	amputation: caudal fin
Observed In:	intestine neutrophil neutrophil neutrophil chemotaxis trunk neutrophil
Stage Range:	Day 6 to Adult

Phenotype Detail

Acknowledgments

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