FIGURE

Fig. S5

ID

ZDB-FIG-191112-10

Publication

Murdoch et al., 2019 - Intestinal Serum amyloid A suppresses systemic neutrophil activation and bactericidal activity in response to microbiota colonization

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Fig. S5

Comparison of Tg(-0.349cldn15la:mCherry) and TgBAC(cldn15la:EGFP)expression patterns.

(A) Widefield fluorescence images of IEC-specific cytosolic mCherry expression in 6 dpf double transgenic Tg(-0.349cldn15la:mCherry)^rdu65; TgBAC(cldn15la:EGFP)^pd1034Tg larvae demonstrated overlap in mCherry and GFP expression domains (scale bar = 500 μm). (B)Representative confocal micrographs of immunolabeled transverse sections from anterior (upper) and posterior (lower) intestinal segments of Tg(-0.349cldn15la:mCherry)^rdu65;TgBAC(cldn15la:EGFP)^pd1034Tg 6 dpf larvae revealed expression of both mCherry and EGFP in IECs (scale bar = 20 μm). (C) Representative maximum intensity projections from single plane illumination microscopy (SPIM) z-stacks of Tg(-0.349cldn15la:mCherry)^rdu65;TgBAC(cldn15la:EGFP) ^pd1034Tg 6 dpf larvae (scale bar = 500 μm). (D-E) High magnification single slice lightsheet images of the intestinal epithelium in a representative 6 dpf Tg(-0.349cldn15la:mCherry)^rdu65;TgBAC(cldn15la:EGFP) ^pd1034Tg larva.

Expression Data

Genes:	GFP mCherry
Fish:	pd1034Tg; rdu65Tg
Anatomical Term:	intestinal epithelial cell
Stage:	Day 6

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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