FIGURE

Fig. 1

ID

ZDB-FIG-190718-33

Publication

Grassini et al., 2019 - Myosin Vb is required for correct trafficking of N-cadherin and cardiac chamber ballooning

Other Figures

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Fig. 1

uq^23ks mutants have chamber ballooning defects. A:Brightfield images of sibling and uq^23ks mutants at 24 and 48 hpf and 4 dpf. Mutants manifest both cardiac (arrowhead) and fin defects (asterisks) at 48 hpf. B: Close up views of the cardiac defect shows the small, dysmorphic heart that does not balloon correctly in mutants and the pericardial cavity is depressed. Fin folds also fail to develop (asterisks) and epithelial cells appear to extrude (arrow). C: ISH assays using myl7and myh6 probes highlight the myocardium, showing that mutants and siblings are indistinguishable at 28 hpf and appears by 36 and 48 hpf. nppastaining at 48 hpf shows no significant differences between siblings and mutants, indicating the myocardium is properly specified and not experiencing mechanical stress.

Expression Data

Genes:	myh6 myl7 nppa
Fish:	WT myo5b^{uq23ks/uq23ks}
Anatomical Terms:	atrial myocardium ventricular myocardium
Stage Range:	Prim-5 to Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	myo5b^{uq23ks/uq23ks}
Observed In:	atrium atrium cardiac chamber ballooning cardiac ventricle cardiac ventricle cardiac chamber ballooning dorsal fin fold dorsal fin fold embryonic dorsal fin morphogenesis dorsal fin fold epithelial cell heart heart contraction ventral fin fold ventral fin fold embryonic anal fin morphogenesis ventral fin fold epithelial cell
Stage Range:	Prim-5 to Day 4

Phenotype Detail

Acknowledgments

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