FIGURE

Fig. 1

ID

ZDB-FIG-190717-24

Publication

Chan et al., 2019 - Development of enhancer-trapping and -detection vectors mediated by the Tol2 transposon in zebrafish

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Fig. 1

Comparison of the enhancer-trapping efficiency of four minimal promoters in zebrafish embryos.

(A) Diagram of the ET vector. The frame contains an ET box flanked by Tol2 TIRs. The ET box contained a minimal promoter, the mCherry ORF and the SV40 polyA. The purple arrow represents the four minimal promoters (Myc, Oct4, Krt4 and Gata), respectively. (B and C) Proportion of mCherry-expression-positive embryos injected with ET vectors at 12 and 72 hpf. Embryos at the one-cell stage were co-injected with ET plasmids (20 pg/nL) mixed with the Tol2 mRNA (30 pg/nL) or were injected with enhancer plasmids (20 pg/nL) alone. (D) mCherry expression in embryos injected with the ET vector carrying the Krt4 minimal promoter at 72 hpf; the white arrow denotes the re-occurring pattern detected in the cerebellum. (E) mCherry expression in embryos injected with the ET vector carrying the Gata minimal promoter at 72 hpf.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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