ZFIN ID: ZDB-FIG-190716-34
Thomas et al., 2019 - Distinct progenitor populations mediate regeneration in the zebrafish lateral line. eLIFE   8 Full text @ Elife
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Condition:
Anatomical Terms:
Stage: Days 7-13
PHENOTYPE:
Fish:
Condition:
Observed In:
Stage: Days 7-13

Fig. 3

Distinct support cell populations have different regenerative capacities.

(A) Timeline of nlsEos fate mapping experiment. Fish were photoconverted at 5 dpf, treated with neomycin, then fixed and imaged 72 hr post treatment (8 dpf). (B, C, D) Maximum projections of neuromasts from sfrp1a:nlsEos (Peripheral, B), tnfsf10l3:nlsEos (AP, C), and sost:nlsEos (DV, D) fish following photoconversion and hair cell regeneration. Converted nlsEos-positive cells are shown in magenta, and brn3c:GFP-positive hair cells are shown in green. Arrowheads indicate nlsEos-positive hair cells. Scale bar = 10 μm. (E) Percentage of hair cells per neuromast labeled by nlsEos following regeneration. Sfrp1a:nlsEos (Peripheral): 3.59 ± 8.87, n = 50 neuromasts (10 fish); tnfsf10l3:nlsEos (AP): 20.28 ± 20.58, n = 50 neuromasts (10 fish); sost:nlsEos (DV): 60.87 ± 12.37, n = 50 neuromasts (10 fish); mean ± SD; Kruskal-Wallis test, Dunn’s post-test, p=0.003 (Peripheral vs. AP), p<0.0001 (Peripheral vs. DV, AP vs. DV). (F) Total nlsEos-positive support cells per neuromast prior to hair cell ablation. Sfrp1a:nlsEos (Peripheral): 14.30 ± 4.17, n = 50 neuromasts (10 fish); tnfsf10l3:nlsEos (AP): 22.8 ± 4.40, n = 50 neuromasts (10 fish); sost:nlsEos (DV): 23.86 ± 4.45, n = 50 neuromasts (10 fish); mean ± SD; Kruskal-Wallis test, Dunn’s post-test, p<0.0001 (Peripheral vs. AP, Peripheral vs. DV), p>0.9999 (AP vs. DV).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
Eos sfrp1aw217Tg; s356tTg chemical treatment by environment: neomycin, chemical ablation: neuromast hair cell Days 7-13 neuromast peripheral region IFL
Days 7-13 neuromast support cell IFL
sostw215Tg; s356tTg chemical treatment by environment: neomycin, chemical ablation: neuromast hair cell Days 7-13 neuromast dorsal region IFL
Days 7-13 neuromast ventral region IFL
Days 7-13 neuromast support cell IFL
tnfsf10l3w218Tg; s356tTg chemical treatment by environment: neomycin, chemical ablation: neuromast hair cell Days 7-13 neuromast anterior region IFL
Days 7-13 neuromast posterior region IFL
Days 7-13 neuromast support cell IFL
GFP sfrp1aw217Tg; s356tTg chemical treatment by environment: neomycin, chemical ablation: neuromast hair cell Days 7-13 neuromast hair cell IFL
sostw215Tg; s356tTg chemical treatment by environment: neomycin, chemical ablation: neuromast hair cell Days 7-13 neuromast hair cell IFL
tnfsf10l3w218Tg; s356tTg chemical treatment by environment: neomycin, chemical ablation: neuromast hair cell Days 7-13 neuromast hair cell IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
sfrp1aw217Tg; s356tTg chemical treatment by environment: neomycin, chemical ablation: neuromast hair cell Days 7-13 neuromast hair cell cell population proliferation increased occurrence, abnormal
Days 7-13 neuromast support cell cell population proliferation increased occurrence, abnormal
sostw215Tg; s356tTg chemical treatment by environment: neomycin, chemical ablation: neuromast hair cell Days 7-13 neuromast hair cell cell population proliferation increased occurrence, abnormal
Days 7-13 neuromast support cell cell population proliferation increased occurrence, abnormal
tnfsf10l3w218Tg; s356tTg chemical treatment by environment: neomycin, chemical ablation: neuromast hair cell Days 7-13 neuromast hair cell cell population proliferation increased occurrence, abnormal
Days 7-13 neuromast support cell cell population proliferation increased occurrence, abnormal
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Elife