ZFIN ID: ZDB-FIG-190716-32
Thomas et al., 2019 - Distinct progenitor populations mediate regeneration in the zebrafish lateral line. eLIFE   8 Full text @ Elife
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: Day 5 to Days 7-13

Fig. 2

Genetic labeling of distinct support cell populations.

(A, C, E) Maximum projections of neuromasts from sfrp1a:nlsEos (Peripheral, A), tnfsf10l3:nlsEos (AP, C), and sost:nlsEos (DV, E) fish. Converted nlsEos-positive cells are shown in magenta, and brn3c:GFP-positive hair cells are shown in green. Scale bar = 10 μm. (B, D, F) Percentage of hair cells per neuromast labeled by Peripheral (B), AP (D), and DV cells (F) at 5 and 8 dpf. (B) 5 dpf: 19.04 ± 19.86, n = 50 neuromasts (10 fish); 8 dpf: 19.46 ± 19.44, n = 50 neuromasts (10 fish); mean ± SD; Mann Whitney U test, p=0.7047. (D) 5 dpf: 5.71 ± 8.22, n = 50 neuromasts (10 fish); 8 dpf: 6.36 ± 9.57, n = 50 neuromasts (10 fish); mean ± SD; Mann Whitney U test, p=0.9668. (F) 5 dpf: 38.93 ± 13.46, n = 50 neuromasts (10 fish); 8 dpf: 55.78 ± 14.13, n = 50 neuromasts (10 fish); mean ± SD; Mann Whitney U test, p<0.0001.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
Eos sfrp1aw217Tg; s356tTg control Day 5 neuromast peripheral region IFL
Day 5 neuromast support cell IFL
Days 7-13 neuromast peripheral region IFL
Days 7-13 neuromast support cell IFL
sostw215Tg; s356tTg control Day 5 neuromast dorsal region IFL
Day 5 neuromast ventral region IFL
Day 5 neuromast support cell IFL
Days 7-13 neuromast dorsal region IFL
Days 7-13 neuromast ventral region IFL
Days 7-13 neuromast support cell IFL
tnfsf10l3w218Tg; s356tTg control Day 5 neuromast anterior region IFL
Day 5 neuromast peripheral region IFL
Day 5 neuromast posterior region IFL
Days 7-13 neuromast anterior region IFL
Days 7-13 neuromast peripheral region IFL
Days 7-13 neuromast posterior region IFL
GFP sfrp1aw217Tg; s356tTg control Day 5 neuromast hair cell IFL
Days 7-13 neuromast hair cell IFL
sostw215Tg; s356tTg control Day 5 neuromast hair cell IFL
Days 7-13 neuromast hair cell IFL
tnfsf10l3w218Tg; s356tTg control Day 5 neuromast hair cell IFL
Days 7-13 neuromast hair cell IFL
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
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