FIGURE

Fig. 2

ID

ZDB-FIG-190716-32

Publication

Thomas et al., 2019 - Distinct progenitor populations mediate regeneration in the zebrafish lateral line

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Fig. 2

Genetic labeling of distinct support cell populations.

(A, C, E) Maximum projections of neuromasts from sfrp1a:nlsEos (Peripheral, A), tnfsf10l3:nlsEos (AP, C), and sost:nlsEos (DV, E) fish. Converted nlsEos-positive cells are shown in magenta, and brn3c:GFP-positive hair cells are shown in green. Scale bar = 10 μm. (B, D, F) Percentage of hair cells per neuromast labeled by Peripheral (B), AP (D), and DV cells (F) at 5 and 8 dpf. (B) 5 dpf: 19.04 ± 19.86, n = 50 neuromasts (10 fish); 8 dpf: 19.46 ± 19.44, n = 50 neuromasts (10 fish); mean ± SD; Mann Whitney U test, p=0.7047. (D) 5 dpf: 5.71 ± 8.22, n = 50 neuromasts (10 fish); 8 dpf: 6.36 ± 9.57, n = 50 neuromasts (10 fish); mean ± SD; Mann Whitney U test, p=0.9668. (F) 5 dpf: 38.93 ± 13.46, n = 50 neuromasts (10 fish); 8 dpf: 55.78 ± 14.13, n = 50 neuromasts (10 fish); mean ± SD; Mann Whitney U test, p<0.0001.

Expression Data

Genes:	Eos GFP
Fish:	sfrp1a^w217Tg; s356tTg sost^w215Tg; s356tTg tnfsf10l3^w218Tg; s356tTg
Anatomical Terms:	neuromast anterior region neuromast dorsal region neuromast peripheral region neuromast posterior region neuromast ventral region neuromast hair cell neuromast support cell
Stage Range:	Day 5 to Days 7-13

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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