FIGURE

Fig. 1

ID

ZDB-FIG-190716-25

Publication

Rocker et al., 2019 - Acute effects of hyperglycemia on the peripheral nervous system in zebrafish ( Danio Rerio) following nitroreductase-mediated β-cell ablation

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Fig. 1

Nitroreductase (NTR)-mediated β-cell ablation induces acute hyperglycemia. A: schematic outline of treatment schedule. MTZ, metronidazole. B and C: treatment of 5-days postfertilization (dpf) ins:NTR fish for 2 days with 10 mM MTZ leads to selective ablation of pancreatic β-cells (C), which are unaffected by DMSO alone (B, red, arrow). D: following the schedule of treatment described in A, there was a significant difference in blood glucose levels in MTZ-treated compared with control fish. *P = 0.0239. E and F: lateral view of non-ins:NTR, Tg(nkx2.2a:megfp); Tg(NBT;DsRed) fish treated with DMSO or DMSO + 10 mM MTZ to determine possible off-target effects of MTZ. NTR, nitroreductase; NBT, neural-specific β-tubulin; megfp, monomeric enhanced green fluorescent protein; DsRed, Discosomared. No differences were observed. Scale bar = 50 μm.

Expression Data

Genes:	DsRed EGFP mCherry
Fish:	jh4Tg vu16Tg; zf148Tg
Condition:	chemical ablation: pancreatic B cell, chemical treatment by environment: metronidazole
Anatomical Terms:	motor neuron axon oligodendrocyte pancreatic B cell
Stage:	Days 7-13

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	jh4Tg
Condition:	chemical ablation: pancreatic B cell, chemical treatment by environment: metronidazole
Observed In:	pancreatic B cell
Stage:	Days 7-13

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Am. J. Physiol. Regul. Integr. Comp. Physiol.