FIGURE

Fig. 4

ID

ZDB-FIG-190716-22

Publication

Rocker et al., 2019 - Acute effects of hyperglycemia on the peripheral nervous system in zebrafish ( Danio Rerio) following nitroreductase-mediated β-cell ablation

Other Figures

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Fig. 4

Acute hyperglycemia affects the integrity of the perineurium. A: in control ins:NTR;Tg(nkx2.2a:megfp) fish 7 days postfertilization (dpf), zonula occludens (ZO)-1⁺ tight junctions are apparent along the motor nerve (A′′, arrow), indicated by their alignment with the perineurium (A′, asterisk). NTR, nitroreductase; megfp, monomeric enhanced green fluorescent protein. B: treatment of 5-dpf ins:NTR;Tg(nkx2.2a:megfp) fish for 2 days in 10 mM metronidazole (MTZ) leads to noticeably absent or attenuated ZO-1⁺ tight junctions (B′′, arrow), even when the perineurium is present (B′, asterisk). Note immaturity of the nkx2.2a⁺ perineurial cells (B′, asterisk). SC, spinal cord; pMN, motor neural progenitor domain. Scale bar = 50 μm. C: intensity of ZO-1 fluorescence was significantly lower in MTZ-treated than control fish. au, Arbitrary units. ****P < 0.0001.

Expression Data

Gene:	EGFP
Antibody:	Ab1-tjp1
Fish:	jh4Tg; vu16Tg
Condition:	chemical ablation: pancreatic B cell, chemical treatment by environment: metronidazole
Anatomical Terms:	oligodendrocyte oligodendrocyte tight junction
Stage:	Days 7-13

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	jh4Tg; vu16Tg
Condition:	chemical ablation: pancreatic B cell, chemical treatment by environment: metronidazole
Observed In:	oligodendrocyte tight junction
Stage:	Days 7-13

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Am. J. Physiol. Regul. Integr. Comp. Physiol.