FIGURE

Fig. 2

ID
ZDB-FIG-190711-16
Publication
Ghersi et al., 2019 - bif1, a new BMP signaling inhibitor, regulates embryonic hematopoiesis in the zebrafish
Other Figures
All Figure Page
Back to All Figure Page
Fig. 2

bif1 is also expressed during definitive and adult hematopoiesis. (A) Schematic indicating the approximate area in the trunk shown in the images in B (dotted rectangle). (B) Confocal images of the trunk region of double-positive bif1:GFP-LT and flk1:mcherry embryos at 36 hpf and 48 hpf. Arrows indicate double-positive cells. (C) Schematic indicating the approximate area in the tail shown in the images in D (rectangle). (D) Confocal images of the CHT of double-positive bif1:GFP-LT and flk1:mcherry embryos at 3 dpf. Arrows indicate double-positive cells. (E) Schematic representing the head of a zebrafish at 4 dpf. The position of the thymus is outlined by the dotted rectangle. (F) Confocal images of thymic area of double-positive bif1:GFP-LT;rag2:DsRed embryos at 4 and 5 dpf. (G) Results from FACS-sorted cells of WKM dissected from 1-year-old embryos. n=3. (H) Expression of bif1 by qPCR on FACS-sorted cells, relative to the WKM. Data were obtained from biological triplicates. SSC, side scatter; FSC, forward scatter; M, myeloid; L/PNE, lymphocytes and progenitors non-erythroid; EP, erythroid progenitors; mRBC, mature red blood cells; WKM, whole kidney marrow.

Expression Data
Genes:
Fish:
Anatomical Term:
Stage Range: Prim-25 to Long-pec

Expression Detail
Antibody Labeling
Phenotype Data

Phenotype Detail
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development