PUBLICATION

bif1, a new BMP signaling inhibitor, regulates embryonic hematopoiesis in the zebrafish

Authors
Ghersi, J.J., Mahony, C.B., Bertrand, J.Y.
ID
ZDB-PUB-190308-4
Date
2019
Source
Development (Cambridge, England)   146(6): (Journal)
Registered Authors
Bertrand, Julien, Ghersi, Joey, Mahony, Christopher
Keywords
BMP signaling, Definitive hematopoiesis, Erythropoiesis, Hematopoietic Stem Cells, Zebrafish, Zinc finger proteins
MeSH Terms
  • Animals
  • Animals, Genetically Modified
  • Bone Morphogenetic Proteins/antagonists & inhibitors*
  • Bone Morphogenetic Proteins/metabolism*
  • Cell Lineage
  • Cell Nucleus/metabolism
  • Erythropoiesis/genetics
  • Gene Expression Regulation, Developmental*
  • Green Fluorescent Proteins/metabolism
  • Hematopoiesis*
  • Mice
  • Signal Transduction
  • Transcription Factors/metabolism
  • Zebrafish
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/physiology*
  • Zinc Fingers
PubMed
30837221 Full text @ Development
Abstract
Hematopoiesis maintains the entire blood system and dysregulation of this process can lead to malignancies (leukemia), immunodeficiencies or red blood cell diseases (anemia, polycythemia vera). We took advantage of the zebrafish model that shares most of the genetic program involved in hematopoiesis with mammals to characterize a new gene of unknown function, si:ch73-299h12.2, expressed in the erythroid lineage during primitive, definitive and adult hematopoiesis. This gene, required during primitive and definitive erythropoiesis, encodes a C2H2 zinc finger protein that inhibits BMP signaling. We therefore named this gene: blood-inducing factor 1 and BMP inhibitory factor 1 (bif1). We identified a bif1 orthologue in Sinocyclocheilus Rhinocerous, another fish, and in the mouse genome. Both genes also inhibit BMP signaling when overexpressed in zebrafish. In conclusion, we deorphanized a new zebrafish gene of unknown function: bif1 codes for a zinc-finger protein that inhibits BMP signaling and also regulates primitive erythropoiesis and definitive hematopoiesis.
Genes / Markers
Figures
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Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes