Fig. 5

Knockdown of Gad1 causes defects in head development and outgrowth visible at 1 dpf (A) wild-type; (B) gad1b splice blocking MO; (C) gad1b translation blocking MO; (D,E) BHQ-gad1b-ccMO; (F) gad1b mismatch control for splice blocking MO; (G,H) CyHQ-gad1b-ccMO; (I) gad1b mismatch control for translation blocking MO. (D,G) BHQ- and CyHQ-gad1b-ccMO injected embryos raised in the dark exhibit morphological development comparable to wild-type embryos. (E, H) BHQ- and CyHQ-gad1b-ccMO injected embryos raised in the light exhibit morphological development comparable to embryos injected with gad1b splice blocking or translation blocking MOs (B–C). Asterisk denotes regions exhibiting altered distribution of the presumptive head mesenchyme; bracket denotes altered gross structure of the midbrain-hindbrain region. Normal development is observed in embryos injected with 5 base mismatch controls for the gad1b splice blocking MO (F) or translation blocking MO (I). Scale bar in (A) applies to all panels. t, telencephalon; e, eye; m, midbrain; h, hindbrain; o, otocyst.