FIGURE

Fig. 7

ID

ZDB-FIG-190627-36

Publication

Yildiz et al., 2019 - Zebrafish prdm12b acts independently of nkx6.1 repression to promote eng1b expression in the neural tube p1 domain

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Fig. 7

prdm12b does not maintain the p1 domain by repressing nkx6.1. a. Anti-Nkx6.1 immunostaining of nkx6.1^um321/um321 mutant (left) and wildtype (right) embryos at 30hpf. b. Chart indicating the frequency of each genotype at various time points in broods from crosses of nkx6.1^+/um321 heterozygous mutants. c. Hb9 immunostaining in wildtype (left) versus a cross of nkx6.1^+/um321 heterozygous embryos (right) at 33hpf. d. Islet-1/2 immunostaining of 50hpf embryos from a cross of nkx6.1^+/um321 heterozygotes. e. Expression of eng1b in 24hpf embryos from a cross of nkx6.1^+/um321 heterozygotes. f. Expression of eng1b in 24hpf uninjected wildtype embryos (left panels), 24hpf prdm12b MO-injected wildtype embryos (middle panels) and 24hpf prdm12b MO-injected embryos from a cross of nkx6.1^+/um321heterozygotes (right panels). g. Expression of prdm12b in a representative wildtype embryo (left panel) and a representative embryo from a cross of nkx6.1^+/um321 heterozygotes (middle panel) at 24hpf. Right panel shows quantification of the size of the prdm12b expression domain in 11 wildtype embryos and 20 embryos from a cross of nkx6.1^+/um321 heterozygotes. Numbers in panels indicate the fraction of embryos displaying the phenotype shown

Expression Data

Genes:	en1b prdm12b
Antibodies:	Ab1-mnx1 Ab2-isl
Fish:	WT nkx6.1^um321/um321 WT + MO1-prdm12b nkx6.1^um321/um321 + MO1-prdm12b
Knockdown Reagent:	MO1-prdm12b
Anatomical Terms:	branchiomotor neuron CiA midbrain hindbrain boundary MiV1 motor neuron somite spinal cord ventral region
Stage Range:	Prim-5 to Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	nkx6.1^um321/um321
Observed In:	branchiomotor neuron whole organism
Stage Range:	Long-pec to Days 45-89

Phenotype Detail

Acknowledgments

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