FIGURE

Fig. 4

ID

ZDB-FIG-190624-6

Publication

Huang et al., 2019 - Rab33a and Rab33ba mediate the outgrowth of forebrain commissural axons in the zebrafish brain

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Fig. 4

The rab33a;rab33ba double mutation inhibits axonal extension in the anterior commissure. (a,c) Representative dorsal views (upper panels) of the anterior commissures immunolabeled with anti-acetylated tubulin antibody and individual DRC neurons expressing tdTomato in wild-type control (a) and rab33a^−/−;rab33ba^−/− double mutant (c) embryos at 36 hpf. The lower panels show only the tdTomato-labeled neurons in the upper panels. The tips of the axons are indicated by arrows. Scale bars: 50 µm. (b,d) The trajectories of individual axons of the tdTomato-labeled DRC neurons in wild-type control (b) and rab33a^−/−;rab33ba^−/− double mutant (e) embryos at 36 hpf. The cell body positions are normalized at (x = 0 μm, y = 0 μm). (e) The length of the axon of DRC neurons in the anterior commissure obtained from the data analyses in (b,d). Axons of wild-type control (n = 9) and rab33a^−/−;rab33ba^−/−double mutant (n = 9) neurons were analyzed at 36 hpf. Data are expressed as mean ± SEM; ^***P < 0.01 (unpaired Student’s t test).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	rab33a^ni103/ni103; rab33ba^ni104/ni104
Observed In:	anterior commissure axon anterior commissure commissural neuron axon guidance dorso-rostral cluster axon extension involved in axon guidance
Stage:	Prim-25

Phenotype Detail

Acknowledgments

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