PUBLICATION
Rab33a and Rab33ba mediate the outgrowth of forebrain commissural axons in the zebrafish brain
- Authors
- Huang, L., Urasaki, A., Inagaki, N.
- ID
- ZDB-PUB-190214-6
- Date
- 2019
- Source
- Scientific Reports 9: 1799 (Journal)
- Registered Authors
- Inagaki, Naoyuki
- Keywords
- none
- MeSH Terms
-
- Animals
- Axons/metabolism*
- Brain/cytology*
- Brain/metabolism*
- Mutation/genetics
- Neurons/metabolism*
- Telencephalic Commissures/cytology*
- Zebrafish
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism*
- rab GTP-Binding Proteins/genetics
- rab GTP-Binding Proteins/metabolism*
- PubMed
- 30755680 Full text @ Sci. Rep.
Citation
Huang, L., Urasaki, A., Inagaki, N. (2019) Rab33a and Rab33ba mediate the outgrowth of forebrain commissural axons in the zebrafish brain. Scientific Reports. 9:1799.
Abstract
Rab small GTPases play key roles in intracellular membrane trafficking. Rab33a promotes axon outgrowth of cultured rat hippocampal neurons by mediating the anterograde axonal transport of Golgi-derived vesicles and the concomitant exocytosis of these vesicles at the growth cone. However, the functions of Rab33 in vivo are unclear. Here, we show that zebrafish rab33a and rab33ba are orthologs of mammalian Rab33a and Rab33b, respectively. They are expressed in the developing brain, including in neurons of the telencephalic dorsorostral cluster and the diencephalic ventrorostral cluster, which project axons to form the anterior and postoptic commissures, respectively. Although rab33a single mutant and rab33ba single mutant fish did not show remarkable defects, fish carrying the rab33a;rab33ba double mutations displayed dysgenesis of the anterior and postoptic commissures. Single-cell labeling in the telencephalic dorsorostral cluster demonstrated that the rab33a;rab33ba double mutation inhibits axonal extension in the anterior commissure. These results suggest that Rab33a and Rab33ba mediate axon outgrowth and the formation of the forebrain commissures in the zebrafish brain in a cooperative manner.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping