ZFIN ID: ZDB-FIG-190624-3
Huang et al., 2019 - Rab33a and Rab33ba mediate the outgrowth of forebrain commissural axons in the zebrafish brain. Scientific Reports   9:1799 Full text @ Sci. Rep.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: 1-cell to Long-pec

Fig. 1

Expression of zebrafish rab33a and rab33ba. (a) RT-PCR analysis of rab33a and rab33ba transcripts. Elongation factor 1a (EF1a) was used as a control. Developmental stages are denoted as hours post-fertilization (hpf). RT-PCR products produced in the presence (RT+) or absence (RT−) of reverse transcriptase were electrophoresed on 2% agarose gels. (b,c) Whole-mount in situ hybridization of rab33a (b) and rab33ba (c) at 24 hpf; (b′) and (c′) show the enlarged lateral (left) and ventral (right) views of the areas indicated by the rectangles. (d,e) Whole-mount in situ hybridization of rab33a (d) and rab33ba(e) at 36 hpf; (d′) and (e′) show the enlarged lateral (left) and ventral (right) views of the areas indicated by the rectangles. Arrowheads and arrows in (b′–e′) indicate the DRC and VRC, respectively. Arrows in (b–e) indicate the hindbrain. See the negative control data for whole-mount in situ hybridization (Supplementary Fig. S3). Scale bars: 100 μm.

 

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
rab33a WT standard conditions 1-cell whole organism RTPCR
Prim-5 dorso-rostral cluster ISH
Prim-5 forebrain ISH
Prim-5 hindbrain ISH
Prim-5 nucleus of the tract of the anterior commissure ISH
Prim-5 nucleus of the tract of the postoptic commissure ISH
Prim-5 ventro-rostral cluster ISH
Prim-5 whole organism RTPCR
Prim-25 dorso-rostral cluster ISH
Prim-25 forebrain ISH
Prim-25 hindbrain ISH
Prim-25 nucleus of the tract of the anterior commissure ISH
Prim-25 nucleus of the tract of the postoptic commissure ISH
Prim-25 ventro-rostral cluster ISH
Prim-25 whole organism RTPCR
Long-pec whole organism RTPCR
rab33ba WT standard conditions 1-cell whole organism RTPCR
Prim-5 dorso-rostral cluster ISH
Prim-5 forebrain ISH
Prim-5 hindbrain ISH
Prim-5 nucleus of the tract of the anterior commissure ISH
Prim-5 nucleus of the tract of the postoptic commissure ISH
Prim-5 ventro-rostral cluster ISH
Prim-5 whole organism RTPCR
Prim-25 dorso-rostral cluster ISH
Prim-25 forebrain ISH
Prim-25 hindbrain ISH
Prim-25 nucleus of the tract of the anterior commissure ISH
Prim-25 nucleus of the tract of the postoptic commissure ISH
Prim-25 ventro-rostral cluster ISH
Prim-25 whole organism RTPCR
Long-pec whole organism RTPCR
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Scientific Reports for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Sci. Rep.