FIGURE

Fig. 4

ID

ZDB-FIG-190624-48

Publication

Sahu et al., 2019 - Neurotrophin receptor Ntrk2b function in the maintenance of dopamine and serotonin neurons in zebrafish

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Fig. 4

Reduced tyrosine hydroxylase immunoreactivity in both the morphants and mutants at 5 dpf. (A) (a–d) TH1 immunohistochemistry revealed a loss of the neuronal population in the diencephalic cluster (5,6,11 cell group) in the Ntrk2b and Ntrk2a morphant. The neuronal cell group affected has been encircled. (A) (a′–d′): Higher magnification images of the affected TH cell group. (B) Total cell counts in the diencephalic region of the morphants. (C) The mutants have reduced TH positive cells in the same cell group and could be rescued by mRNA overexpression. (C) (a′–d′) Higher magnification images of the affected TH cell group. (D) TH cell counts from the mutants in the 5,6,11 cell group. (Bars represent Mean ± SEM, one-way ANOVA, Kruskal-Wallis test **p-value < 0.008, ****p-value < 0.0002, Scale bar = 100 um).

Expression Data

Gene:	th
Antibody:	Ab1-th
Fish:	WT ntrk2b^{sa13660/sa13660} WT + MO1-ntrk2a WT + MO2-ntrk2b
Knockdown Reagents:	MO1-ntrk2a MO2-ntrk2b
Anatomical Term:	diencephalon dopaminergic neuron
Stage:	Day 5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	ntrk2b^{sa13660/sa13660} WT + MO1-ntrk2a WT + MO2-ntrk2b
Knockdown Reagents:	MO1-ntrk2a MO2-ntrk2b
Observed In:	diencephalon dopaminergic neuron diencephalon ventral region
Stage:	Day 5

Phenotype Detail

Acknowledgments

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