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Fig. 4
Reduced tyrosine hydroxylase immunoreactivity in both the morphants and mutants at 5 dpf. (A) (a–d) TH1 immunohistochemistry revealed a loss of the neuronal population in the diencephalic cluster (5,6,11 cell group) in the Ntrk2b and Ntrk2a morphant. The neuronal cell group affected has been encircled. (A) (a′–d′): Higher magnification images of the affected TH cell group. (B) Total cell counts in the diencephalic region of the morphants. (C) The mutants have reduced TH positive cells in the same cell group and could be rescued by mRNA overexpression. (C) (a′–d′) Higher magnification images of the affected TH cell group. (D) TH cell counts from the mutants in the 5,6,11 cell group. (Bars represent Mean ± SEM, one-way ANOVA, Kruskal-Wallis test **p-value < 0.008, ****p-value < 0.0002, Scale bar = 100 um).