ZFIN ID: ZDB-FIG-190618-7
Young et al., 2019 - Compensatory growth renders Tcf7l1a dispensable for eye formation despite its requirement in eye field specification. eLIFE   8 Full text @ Elife
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Anatomical Terms:
Stage Range: 1-cell to Bud
PHENOTYPE:
Fish:
Observed In:
Stage Range: 90%-epiboly to Long-pec

Fig. 7

Ztcf7l1a-/- mutants lacking Hesx1 function fail to form eyes.

(A) Schematic of the genetic strategy to isolate mutations that modify the tcf7l1a-/- mutant phenotype. (B–CU910 modifier of the tcf7l1a-/- mutant phenotype. Lateral views of homozygous U910 embryos that are heterozygous (B) or homozygous (C) for the tcf7l1amutation. (D) Representation of SSLP segregation linkage analysis mapping of U910 modifier of tcf7l1a to a 1.46 megabase (Mb) interval on chromosome 11 (Ch11; rec, recombinants). (E) RT-PCR for hesx1 spanning exons 1–2 (top panel), exons 2–3 (middle panel) and β-actin (bottom panel) on wildtype (lanes 1 and 2) and U910-/- (lanes 3 and 4) embryo cDNA from 1 cell stage (lanes 1 and 3) and 10hpf (lanes 2 and 4). Single experiment. (F–Ihesx1 in situhybridisation on wildtype (F–H) and U910-/- (I) embryos at epiboly (ep) stages indicated. Dorsal views, anterior up. (J, K) Lateral views of hesx1-/- (Δex1/2)/tcf7l1a+/- (J) and hesx1-/-(Δex1/2)/Ztcf7l1a-/- (K) embryos. Four independent experiments, n = 53. Scale bars = 200 µm.

 

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
hesx1 WT standard conditions 1-cell Not Detected whole organism RTPCR
Shield anterior presumptive neural plate ISH
75%-epiboly anterior neural plate ISH
90%-epiboly anterior neural plate ISH
Bud whole organism RTPCR
hesx1u910/u910 standard conditions 1-cell Not Detected whole organism RTPCR
90%-epiboly Not Detected anterior neural plate ISH
Bud Not Detected whole organism RTPCR
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
hesx1u910/u910 standard conditions 90%-epiboly anterior neural plate hesx1 expression absent, abnormal
Bud whole organism hesx1 expression absent, abnormal
tcf7l1am881/m881 ; hesx1u910/u910 standard conditions Long-pec eye absent, abnormal
Acknowledgments:
ZFIN wishes to thank the journal eLIFE for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Elife