FIGURE

Fig. 2

ID

ZDB-FIG-190604-20

Publication

Ahmed Alfar et al., 2017 - Distinct Levels of Reactive Oxygen Species Coordinate Metabolic Activity with Beta-cell Mass Plasticity

Other Figures

All Figure Page

Back to All Figure Page

Fig. 2

Catalase expression in beta-cells reduces beta-cell proliferation. (A) Confocal sections of principal islets from WT and Tg(ins:catalase;ins:H2BmCherry) siblings, incubated at 21 dpf with EdU for 16 hours (5 h after feeding). Fewer beta-cells incorporated EdU in Tg(ins:catalase;ins:H2BmCherry) compared to WT siblings. (B) Quantification of the percentage of EdU⁺ beta-cells in WT (n = 10) and Tg(ins:catalase;ins:H2BmCherry) (n = 11). Expression of catalase leads to a reduced percentage of EdU⁺ beta-cells compared to WT (p = 0,001). (C) Confocal planes (xzy) and 3D reconstructions (xzy) of smaller islets from WT and Tg(ins:catalase;ins:H2BmCherry) animals stained for insulin and glucagon. The beta-cells in the islet of the Tg(ins:catalase;ins:H2BmCherry) were scattered and fewer in numbers compared to WT. Alpha-cells in Tg(ins:catalase;ins:H2BmCherry) appear unaffected by the catalase expression in beta-cells. See also Figure S2 for maximum projections spanning the volume of principal islets in WT and Tg(ins:catalase;ins:H2BmCherry). Error bars = SEM.

Expression Data

Gene:	mCherry
Fish:	tud206Tg
Anatomical Term:	pancreatic B cell
Stage:	Days 21-29

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	tud206Tg
Observed In:	pancreatic A cell pancreatic B cell pancreatic B cell cell population proliferation primary islet pancreatic B cell
Stage:	Days 21-29

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Sci. Rep.