FIGURE

Fig. 2

ID

ZDB-FIG-190114-8

Publication

López Nadal et al., 2018 - Exposure to Antibiotics Affects Saponin Immersion-Induced Immune Stimulation and Shift in Microbial Composition in Zebrafish Larvae

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Fig. 2

Effect of saponin immersion on zebrafish larvae. (A) Percent survival of zebrafish exposed to control (E3), 0.5 mg/ml saponin, 0.7 mg/ml saponin and 1 mg/ml saponin from 6 - 9 dpf (n = 40 fish / treatment) (Log-rank Mantel-Cox Test for Chi-square, ^???p < 0.0005). (B) Representative pictures of the saponin-treated Tg(mpeg1:mCherry-Fump2/mpx:eGFPi114) fish displaying green neutrophils and red macrophages. (C) Quantification of neutrophils and macrophages in the intestinal area (n = 6?11 fish / group) (one way ANOVA Kruskal-Wallis test with Dunn?s Multiple comparison Post-Test, mean ± SEM, ^?p < 0.05 ^??p < 0.01). Top: counted cells in intestinal area. Bottom: Corrected Total Cell Fluorescence (CTCF, measure for total fluorescent pixels in the whole fish). Two independent experiments were performed and data are combined.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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