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Fig. 1

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ZDB-FIG-190108-42

Publication

Meireles et al., 2018 - The Lysosomal Transcription Factor TFEB Represses Myelination Downstream of the Rag-Ragulator Complex

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Fig. 1

rraga Is Essential for CNS Myelination and Acts Autonomously in Oligodendrocytes

(A–E) Analysis of mbp or plp1b mRNA expression at 5 dpf by whole mount in situ hybridization.

(A) Compared to their wild-type siblings, rraga^−/− mutants show reduced mbp expression in the CNS (white arrows), whereas mbp expression in the PNS is normal (black arrows).

(B) Expression of plp1b is also reduced in rraga^−/− mutants. See also Figure S1.

(C) mtor^−/− mutants are developmentally delayed and have a small reduction in mbp mRNA expression levels in the CNS and PNS. See also Figure S2.

(D) Quantification of rescue of mbp expression in rraga^−/−mutants following expression of wild-type rraga under the control of the claudink, mpeg (expressed in macrophages), or huc promoter (expressed in neurons).

(E) Whole mount in situ hybridization analysis of mbp mRNA expression in 5-dpf rraga^−/− mutants following expression of wild-type rraga under the control of different tissue-specific promoters. mbp mRNA expression is partially or fully rescued only when wild-type rraga is expressed in oligodendrocytes (claudink promoter).

All panels show dorsal views, with anterior to the top. Genotypes of all animals shown were determined by PCR after imaging. Scale bar, 50 μm.

Expression Data

Genes:	mbpa plp1b
Fish:	TL mtor^{xu015Gt/xu015Gt} (TL) rraga^st77/st77 (TL)
Anatomical Terms:	central nervous system oligodendrocyte peripheral nervous system myelinating Schwann cell
Stage:	Day 5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	mtor^{xu015Gt/xu015Gt} (TL) rraga^st77/st77 (TL)
Observed In:	central nervous system central nervous system myelination central nervous system oligodendrocyte embryo development peripheral nervous system myelinating Schwann cell peripheral nervous system myelination in peripheral nervous system
Stage:	Day 5

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Cell, 47, Meireles, A.M., Shen, K., Zoupi, L., Iyer, H., Bouchard, E.L., Williams, A., Talbot, W.S., The Lysosomal Transcription Factor TFEB Represses Myelination Downstream of the Rag-Ragulator Complex, 319-330.e5, Copyright (2018) with permission from Elsevier. Full text @ Dev. Cell