ZFIN ID: ZDB-FIG-181207-69
Teng et al., 2018 - Altered bone growth dynamics prefigure craniosynostosis in a zebrafish model of Saethre-Chotzen syndrome. eLIFE   7 Full text @ Elife
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Antibody:
Fish:
Anatomical Terms:
Stage: Days 21-29
PHENOTYPE:
Fish:
Observed In:
Stage: Days 21-29

Fig. 5

Altered proliferation and osteoblast production at mutant zebrafish bone fronts.

(A) Dissected skullcaps were stained for BrdU (magenta) and Sp7 protein (green) at 9 mm. Top panels show maximum intensity projections of whole skull volumes, and middle panels are the same volumes but processed to extract the bone fronts (note that much of the BrdU staining in the center of the top images is in the skin). Bottom panels show enlarged regions of the osteogenic fronts (dotted rectangles). White arrows show proliferative osteoblasts (BrdU+/Sp7+) and magenta arrows show adjacent proliferative Sp7- cells. fb, frontal bone; pb, parietal bone. Scale bars, 300 µm for whole skull view, 100 µm for enlarged view. (B, C) Based on the extracted osteogenic fronts (middle panels in A), we quantified the percentage of Sp7+ osteoblasts that were BrdU+ (B) and the number of adjacent BrdU+/Sp7- cells per area (C). Wild-type controls, n = 20; tcf12; twist1b mutants, n = 28. p values were determined by a Student’s t-test; error bars represent standard error of the mean.

Gene Expression Details No data available
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-sp7 IHC b1212Tg standard conditions Days 21-29 cranial vault osteoblast
IHC Days 21-29 frontal bone osteoblast
IHC Days 21-29 parietal bone osteoblast
IHC twist1bel570/el570 ; tcf12el548/el548 ; b1212Tg standard conditions Days 21-29 cranial vault osteoblast
IHC Days 21-29 frontal bone osteoblast
IHC Days 21-29 parietal bone osteoblast
Phenotype Details
Fish Conditions Stage Phenotype
twist1bel570/el570 ; tcf12el548/el548 ; b1212Tg standard conditions Days 21-29 frontal bone bone growth increased occurrence, abnormal
Days 21-29 frontal bone osteoblast proliferation increased occurrence, abnormal
Days 21-29 parietal bone bone growth increased occurrence, abnormal
Days 21-29 parietal bone osteoblast proliferation increased occurrence, abnormal
Acknowledgments:
ZFIN wishes to thank the journal eLIFE for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Elife