FIGURE

Fig. 4

ID

ZDB-FIG-181206-5

Publication

Yang et al., 2018 - Hypoxia Delays Oligodendrocyte Progenitor Cell Migration and Myelin Formation by Suppressing Bmp2b Signaling in Larval Zebrafish

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Fig. 4

The Bmp2b inhibitor suppresses OPC migration to the dorsal area. (A,B) qRT-PCR analysis indicated that bmp2b gene mRNA expression was significantly downregulated after hypoxia and CoCl₂ exposure. (C,D) Bmp2b protein expression was analyzed with western blots. The results showed that hypoxia blocked Bmp2b protein translation. Thirty zebrafish 4-dpf larvae were pooled for western blots experiments. (E,F) The number of dorsally migrated olig2⁺ cells at 3 and 4 dpf decreased after LDN193189 treatment. E1?, E2?, E3?, and E4? are magnified pictures of the white boxes in E1, E2, E3, and E4. Scale bars: E1?E4, 50 ?m and E1??E4?, 10 ?m. Two-way ANOVA, P < 0.0001: Student?s two-tailed t-test. Significantly more cells were dorsally migrated in 3-dpf control larvae as compared to 3-dpf larvae treated with 10 ?M LDN193189 (n = 12; P < 0.0001). Significantly more cells were dorsally migrated in 4-dpf normoxic larvae as compared to 4-dpf larvae treated with 10 ?M LDN193189 (n = 10; P < 0.0001). ^?P < 0.05; ^??P < 0.01; ^???P < 0.001; ^????P < 0.0001. Error bars represent S.E.M.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front. Cell. Neurosci.