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General Information
ZIRC
ZFIN ID: ZDB-FIG-181130-25
Castillo-Robles et al., 2018 - smarce1 mutants have a defective endocardium and an increased expression of cardiac transcription factors in zebrafish. Scientific Reports   8:15369 Full text @ Sci. Rep.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Anatomical Terms:
Stage: Protruding-mouth
PHENOTYPE:
Fish:
Observed In:
Stage Range: Protruding-mouth to Day 4

Fig. 2

Morphological defects in smarce1 mutants. (A) The heart morphological defects of mutated embryo can be appreciated in comparison with a heart from a normal sibling (arrow) at 72 hpf. B-C. Sagittal sections stained with hematoxylin-eosin from 96 hpf normal (B) and mutant (C) embryos. (D–F) Confocal Z-projections of cmlc2:GFP/smarce1 normal (D) and defective hearts (E,F) at 72 hpf. In F nuclei were stained with DAPI. A: atria, AVC: atrio-ventricular canal, EC: endocardium, Ht: heart, V: ventricle. Scale bars: 25 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP smarce1una1/una1 ; twu34Tg standard conditions Protruding-mouth atrial myocardium IHC
Protruding-mouth ventricular myocardium IHC
twu34Tg standard conditions Protruding-mouth atrial myocardium IHC
Protruding-mouth ventricular myocardium IHC
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
smarce1una1/una1 standard conditions Day 4 atrioventricular canal morphogenesis decreased occurrence, abnormal
Day 4 heart tubular, abnormal
Day 4 heart unlumenized, abnormal
Day 4 heart tube formation decreased occurrence, abnormal
smarce1una1/una1 ; twu34Tg standard conditions Protruding-mouth atrium cardiac muscle cell condensed, abnormal
Protruding-mouth atrium cardiac muscle cell disorganized, abnormal
Protruding-mouth cardiac ventricle cardiac muscle cell condensed, abnormal
Protruding-mouth cardiac ventricle cardiac muscle cell disorganized, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Scientific Reports for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Sci. Rep.