FIGURE

Fig. 2

ID

ZDB-FIG-181128-1

Publication

Ashlin et al., 2018 - Pitpnc1a Regulates Zebrafish Sleep and Wake Behavior through Modulation of Insulin-like Growth Factor Signaling

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Fig. 2

pitpnc1a ^-/- Larvae Are Behaviorally Hyperactive

(A) CRISPR/Cas9 generated a 5-base pair deletion in exon 2 of pitpnc1a.

(B) Pitpnc1a protein is detected in pitpnc1a^+/+ brain lysates, weaker in pitpnc1a^+/-, and undetectable in pitpnc1a^-/-.

(C) An activity plot across a 14:10 hr day-night cycle (zoomed in C′) reveals hyperactivity in 6 dpf pitpnc1a^-/-. The shaded ribbons represent ± SEM.

(D and E) Bar plots (±SEM) of the mean day (D) and night (E) activity. pitpnc1a^-/- animals are significantly more active during both day and night (one-way ANOVA, Tukey’s post hoc test).

(F) At 36 hpf, pitpnc1a^-/- embryos twitch significantly more than pitpnc1a^+/+ (mean ± SEM, one-way ANOVA).

(G) At both 36 and 52 hpf, pitpnc1a^-/- embryos are significantly more touch sensitive (pooled data from 5 and 4 independent experiments, respectively, and repeated in a blinded pitpnc1a^+/- in-cross; chi-square test).

Expression Data

Gene:	pitpnc1a
Antibody:	Ab1-pitpnc1a
Fish:	AB/TL pitpnc1a^u506/u506 (AB/TL) pitpnc1a^u506/+ (AB/TL)
Anatomical Term:	brain
Stage:	Adult

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	pitpnc1a^u506/u506 (AB/TL) pitpnc1a^u506/+ (AB/TL)
Observed In:	brain musculoskeletal movement thigmotaxis
Stage Range:	Prim-25 to Adult

Phenotype Detail

Acknowledgments

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