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Fig. 2
pitpnc1a -/- Larvae Are Behaviorally Hyperactive
(A) CRISPR/Cas9 generated a 5-base pair deletion in exon 2 of pitpnc1a.
(B) Pitpnc1a protein is detected in pitpnc1a+/+ brain lysates, weaker in pitpnc1a+/-, and undetectable in pitpnc1a-/-.
(C) An activity plot across a 14:10 hr day-night cycle (zoomed in C′) reveals hyperactivity in 6 dpf pitpnc1a-/-. The shaded ribbons represent ± SEM.
(D and E) Bar plots (±SEM) of the mean day (D) and night (E) activity. pitpnc1a-/- animals are significantly more active during both day and night (one-way ANOVA, Tukey’s post hoc test).
(F) At 36 hpf, pitpnc1a-/- embryos twitch significantly more than pitpnc1a+/+ (mean ± SEM, one-way ANOVA).
(G) At both 36 and 52 hpf, pitpnc1a-/- embryos are significantly more touch sensitive (pooled data from 5 and 4 independent experiments, respectively, and repeated in a blinded pitpnc1a+/- in-cross; chi-square test).