Fig. S11

Canonical Wnt signaling activity in lens epithelium is inhibited in rw341 mutants

(A) dGFP mRNA expression (blue) in Tg[TOP:dGFP] transgenic wild-type lenses at 2 and 3 dpf. dGFP mRNA is expressed in the peripheral region of lens epithelium (arrows) as well as pigment epithelium associated with retinal CMZ (arrowheads).

(B) dGFP protein expression (green) of wild-type and rw341 mutant lenses carrying the transgene Tg[TOP:dGFP] at 5 dpf. Nuclei were counter-stained with TOPRO (white). Middle panels indicate only the green channel. Right panels show higher magnification of the anterior lens area. White dotted lines indicate lens epithelium in wild-type and multilayered anterior lens cells in rw341 mutants. The yellow dotted line indicates the peripheral edge of the posterior lens fiber core. Yellow arrows indicate dGFP signals in early differentiating lens fiber cells in wild-type lenses (middle panels). Very faint dGFP signals were detected in peripheral lens epithelium corresponding to the germinative zone in wild-type lenses (white arrows in the right panels), where dGFP mRNA is expressed (A). There is no dGFP signal in anterior multilayered lens cells (white asterisk) and posterior lens fiber area (yellow asterisk) in rw341 mutants. Scale bars: 20 μm.