ZFIN Figure: Powell et al., 2017, Fig. 1

Fig. 1

Cxcr1 Is Required for Neutrophil Recruitment to Tissue Wounds, whereas Cxcr2 Is Required for Neutrophil Resolution

(A) Sudan black staining of 3 days post-fertilization (dpf) larvae at 1, 3, and 6 hr post-wound (hpw) reveals Cxcr1−/− larvae recruit less neutrophils to tail-transection compared to Cxcr1+/− control.

(B) Quantification of neutrophils caudal to the notochord (n = 16 heterozygote [het] and 19 mutant larvae [1 hpw], 18 het and 18 mutant [3 hpw], and 25 het and 21 mutant [6 hpw]).

(C) Quantification of total neutrophils in 3 dpf Cxcr1+/− and Cxcr1−/− larvae (n = 19 het and 23 mutant).

(D) Sudan black staining of 3 dpf larvae at 3 and 6 hpw show more neutrophils at the wound microenvironment in Cxcr2−/− embryos than WT.

(E) Quantification of neutrophils caudal to the notochord in Cxcr2−/− and WT (n = 70 WT and 46 mutant [3 hpw] and 83 WT and 54 mutant [6 hpw]).

(F) Total neutrophil number is higher in Cxcr2−/− than WT (n = 48 WT and 40 mutant larvae).

Error bars represent SE. ^∗p < 0.05.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	cxcr1^uwm33/uwm33 cxcr2^uwm35/uwm35
Condition:	transection: caudal fin
Observed In:	regenerating fin neutrophil whole organism neutrophil
Stage:	Protruding-mouth

Phenotype Detail

Acknowledgments

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