FIGURE

Fig. 3

ID

ZDB-FIG-180827-75

Publication

Saydmohammed et al., 2018 - A High-Content Screen Reveals New Small-Molecule Enhancers of Ras/Mapk Signaling as Probes for Zebrafish Heart Development

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Fig. 3

ST006994, ST020101, and ST011282 treatment induced the dorsalization and expansion of cardiac progenitor cells in zebrafish, which is a Fgf hyperactivation phenotype. (A) Semi-quantitative RT-PCR showing an increase of dusp6 and EGFP transcripts after chemical treatment. (B) Representative images of whole mount in situ hybridization showing tbxta, chordin, and pax2a/egr2b expression after compound treatment. tbxta expression was increased in the tailbud (blue arrow), and chordin expression was expanded in the dorsal region when compared with DMSO control (distance between two red arrows). The neural markers pax2a and egr2b showed lateral expansion (red brackets), which is indicative of dorsalized phenotype from increased early Fgf activity.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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