FIGURE

Fig. 2

ID

ZDB-FIG-180827-74

Publication

Saydmohammed et al., 2018 - A High-Content Screen Reveals New Small-Molecule Enhancers of Ras/Mapk Signaling as Probes for Zebrafish Heart Development

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Fig. 2

In vivo whole organism chemical screen identified new hyperactivators of Fgf/Ras/Mapk signaling. (A) Representative fluorescence micrographs of Tg(dusp6:EGFP) embryos (30 hpf) treated at 24 hpf for 6 h. Top panel, original scan image; bottom panel, images with Cognition Network Technology (CNT) algorithm applied. All of the compounds listed above images were at 10 µM. (B) Confirmatory dose response assay using repurchased small molecules: ST06994, ST011282, and ST020101. Each data point represents an independent assessment of total green fluorescent protein (GFP) intensity in the head of zebrafish larvae (n = 8). Horizontal bars denote the mean ± S.D. from two or three independent experiments.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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