ZFIN Image: Saydmohammed et al., 2018, Fig. 3

Image

Figure Caption

Fig. 3

ST006994, ST020101, and ST011282 treatment induced the dorsalization and expansion of cardiac progenitor cells in zebrafish, which is a Fgf hyperactivation phenotype. (A) Semi-quantitative RT-PCR showing an increase of dusp6 and EGFP transcripts after chemical treatment. (B) Representative images of whole mount in situ hybridization showing tbxta, chordin, and pax2a/egr2b expression after compound treatment. tbxta expression was increased in the tailbud (blue arrow), and chordin expression was expanded in the dorsal region when compared with DMSO control (distance between two red arrows). The neural markers pax2a and egr2b showed lateral expansion (red brackets), which is indicative of dorsalized phenotype from increased early Fgf activity.

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Molecules