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Hsa.CTGF-based zebrafish transgenic lines are bona fide Yap1/Taz reporter. (A) Morpholino-mediated Yap1/Taz knockdown reduces Hsa.CTGF reporter signal (in red). Two splice morpholinos, targeting respectively Yap1 and Taz pre-mRNAs, were co-injected in one-cell stage Tg(Hsa.CTGF:nlsmCherry)ia49 embryos. (B?D) Constitutive activation of Yap1/Taz-mediated transcription increases Hsa.CTGF reporter signal (in red). The mRNA coding for constitutively active versions of YAP1 (B), TAZ (C) or TEAD (D) were injected in one-cell stage Tg(Hsa.CTGF:nlsmCherry)ia49 embryos. The fluorescent reporter expression was documented and quantified at 24 hpf. Control morpholino-injected: n?=?20; Yap/Taz morpholino-injected: n?=?29; controls for YAP-5SA: n?=?12; YAP-5SA mRNA-injected: n?=?12; controls for TAZ-4SA: n?=?57; TAZ-4SA mRNA-injected: n?=?56; controls for TEAD-VP16: n?=?20; TEAD-VP16 mRNA-injected: n?=?20. A.U.: arbitrary units; *p?<?0.05; ***p?<?0.001.
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