FIGURE

Fig. 2

ID

ZDB-FIG-180824-28

Publication

Gao et al., 2018 - IDH1-mutated transgenic zebrafish lines: An in-vivo model for drug screening and functional analysis

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Fig. 2

Characterization of Gfap zebrafish lines.

Expression of transgene was detected using fluorescent imaging (A), immunohistochemistry with an anti-GFP antibody (B) and QPCR (C). Elevated levels of D2HG were only detected in Gfap^R132C lines (D). L2HG levels in the transgenic fish embryos were not affected (E). About 21% Gfap^R132C embryos showed defects in tail development on 1dpf (F and G). Arrow heads: the site of abnormal tail development in the Gfap^R132C embryos. For Q-PCR experiments, we used a pool of five (3dpf) or three (20 dpf) fish per time-point; D2HG and L2HG measurements were averages of two replicates using five (3 and 5 dpf) or three (20 dpf) fish per replicate. Scale bar: 500 μm.

Expression Data

Gene:	EGFP
Fish:	zf3040Tg/zf3040Tg (TL) zf3036Tg/zf3036Tg (TL) zf3038Tg/zf3038Tg (TL) zf3039Tg/zf3039Tg (TL)
Anatomical Terms:	brain spinal cord
Stage Range:	Prim-5 to Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	zf3038Tg/zf3038Tg (TL)
Observed In:	whole organism (R)-2-hydroxyglutarate(2-) whole organism posterior region
Stage Range:	Prim-5 to Protruding-mouth

Phenotype Detail

Acknowledgments

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