ZFIN ID: ZDB-FIG-180824-2
Corral-Serrano et al., 2018 - C2orf71a/pcare1 is important for photoreceptor outer segment morphogenesis and visual function in zebrafish. Scientific Reports   8:9675 Full text @ Sci. Rep.
EXPRESSION / LABELING:
Antibodies:
Fish:
Anatomical Terms:
Stage Range: Day 5 to Adult
PHENOTYPE:
Fish:
Observed In:
Stage Range: Day 5 to Adult

Fig. 3

Pcare1rmc100/rmc100 zebrafish show aberrant photoreceptor morphology. (A) Analysis of the morphology of the pcare1rmc100/rmc100 zebrafish larval retina (5 dpf) using boron-dipyrromethene (BODIPY) revealed disorganization of photoreceptor outer segments as compared to those of strain- and age-matched wild-type larvae (arrows). (B) Siblings of adult zebrafish without (wild-type) or containing (pcare1rmc100/rmc100) the 29 bp deletion in pcare1 were sectioned and stained with antibodies against F-actin (red), Rhodopsin (green) or, in (C) GNAT2 (green). Arrows indicate normal outer segments in control fish and dysmorphic outer segments in mutant fish. Nuclei were stained with DAPI.

Gene Expression Details No data available
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab2-gnat2 IHC WT standard conditions Day 5 retinal cone cell
IHC Adult retinal cone cell
IHC pcare1rmc100/rmc100 standard conditions Day 5 retinal cone cell
IHC Adult retinal cone cell
Ab5-rho IHC WT standard conditions Day 5 retinal rod cell
IHC Adult retinal rod cell
IHC pcare1rmc100/rmc100 standard conditions Day 5 retinal rod cell
IHC Adult retinal rod cell
Phenotype Details
Fish Conditions Stage Phenotype
pcare1rmc100/rmc100 standard conditions Day 5 photoreceptor outer segment layer disorganized, abnormal
Day 5 photoreceptor outer segment layer morphology, abnormal
Adult optomotor response decreased process quality, abnormal
Adult photoreceptor inner segment layer decreased thickness, abnormal
Adult photoreceptor outer segment layer decreased thickness, abnormal
Adult photoreceptor outer segment layer disorganized, abnormal
Adult photoreceptor outer segment layer morphology, abnormal
Adult visual behavior decreased process quality, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Scientific Reports for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Sci. Rep.