FIGURE

Fig. 1

ID

ZDB-FIG-180821-13

Publication

McMillan et al., 2018 - A regulatory pathway involving retinoic acid and calcineurin demarcates and maintains joint cells and osteoblasts in the fin regenerate

Other Figures

All Figure Page

Back to All Figure Page

Fig. 1

Periodic formation of a cluster of joint-forming cells during fin regeneration. (A-A′) Bright-field images of fin regenerate at 3 dpa (A) and 4 dpa (A′) illustrate the periodic addition of joints (yellow arrowheads) to the distal end of the rays. Dashed yellow line indicates amputation plane. (B) DAPI staining on a 4 dpa regenerate longitudinal cryosection illustrates a cluster of nuclei (yellow arrowheads) 232 μm (yellow bracket) from a mature joint. (B′) Magnification of the boxed area in B showing the nuclei cluster (yellow circle). (C) Zns5 immunohistostaining labels the joint cell cluster and adjacent osteoblasts. (C′) DAPI staining of the same section showing the cell cluster nuclei. (C″) Merged image of C and C′. b, blastema; e, epidermis; l, lepidotrichia; o, osteoblast. Scale bars: 200 μm (A,A′); 50 μm (B); 10 μm (B′; in C for C-C″).

Expression Data

Antibody:	zns-5
Fish:	WT
Condition:	amputation: caudal fin
Anatomical Terms:	regenerating fin joint regenerating fin osteoblast
Stage:	Adult

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development