FIGURE

Fig. 4

ID

ZDB-FIG-180809-7

Publication

Malhotra et al., 2018 - Spatio-temporal regulation of concurrent developmental processes by generic signaling downstream of chemokine receptors

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Fig. 4

Control of dorsoventral axis specification by different chemokine receptors.

(A) Epifluorescence image of 5 hpf ccr7-/- mutant embryos carrying the gsc::gfp transgene. Uninjected embryo (left panel) and embryos sensitized by injection of Δβ-Catenin encoding RNA (right panels) are presented. The embryos were also injected with control RNA (cntl) or with RNA encoding for different chemokine receptors (Cxcr4a, Cxcr4b or Ccr9) and their cognate ligands (Cxcl12b, Cxcl12a or Ccl25). (B–D) Graphs showing the area of the goosecoid expression domain determined by quantifying the number of pixels with GFP signal above the auto threshold in the different treatments. 100 pg of mRNA encoding for the receptors was injected and 60 pg of RNA encoding for the ligands. 2.5 pg of Δβ-Catenin-encoding RNA was inject to sensitize the embryos. Equimolar amounts of control RNA were used. For raw data see Figure 4—source data 1.

Expression Data

Gene:	GFP
Fish:	ccr7^stl7/stl7; ml1Tg
Anatomical Term:	whole organism dorsal region
Stage:	30%-epiboly

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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