Fig. 3
- ID
- ZDB-FIG-180809-5
- Publication
- Malhotra et al., 2018 - Spatio-temporal regulation of concurrent developmental processes by generic signaling downstream of chemokine receptors
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CC and CXC receptors can direct the migration of primordial cells. The experimental scheme is provided at the top. (A) Epifluorescence image of 10 hpf embryos expressing the control RNA or RNA encoding for the indicated ligand (Cxcl12a, Cxcl12b, Ccl25 and Ccl19) in one half of the embryo and control RNA or Receptor-encoding RNA (Cxcr4b, Cxcr4a, Ccr9 or Ccr7) in PGCs. Merged images show the position of PGCs with respect to control or ligand-expressing domains (red). (B–D) Graphs show the quantitation of the migration of PGCs as the percentage of GFP-labeled cells located within the ligand-expressing part of the embryos. 60 pg of mGFP-nanos was used to label PGCs in green and 40 pg of m-cherry mRNA was used for labeling the ligand expressing half of the embryo. 20 pg of receptor-encoding RNA was used and 30 pg of ligand-encoding RNA was used. 0.2 pmol of Cxcl12a morpholino was used. Equimolar amounts of control RNA were used. For raw data see Figure 3—source data 1. |