Fig. 3

Effect of C7aSP on hC7aSPs and bC7aSP liver and intravascular lipids through Oil Red O (ORO) staining. (A–E) Lateral view of the whole-mount ORO staining of representative larvae. Enlargement at the trunk and posterior level is shown in panels ′ and ″. WT larva fed with LFD (A) and with HDF (B). hC7aSP1 larva fed with LFD (C). hC7aSP2 larva fed with LFD (D). bC7aSP1 larva fed with LFD (E). (F) The percentages of hC7aSPs and bC7aSP larvae with steatosis and intravascular lipids was calculated through ORO staining. The ORO staining was performed in triplicate with on average 60–80 larvae per groups. The asterisk represents statistically significant differences; * p < 0.01, and ** p < 0.005. Abbreviations: e, eye; da, dorsal aorta; dlav, dorsal longitudinal anastomotic vessel; h, heart; i, intestine; isv, intersegmental vessel; pcv, posterior cardinal vein; sb, swim bladder; ys, yolk sac. Scale bar, 200 μm in panels A–E; 50 μm in panels A′–E′; 100 μm in panels A″–E″.