FIGURE

Fig. 3

ID

ZDB-FIG-180709-4

Publication

Dobrzycki et al., 2018 - An optimised pipeline for parallel image-based quantification of gene expression and genotyping after in situ hybridisation.

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Fig. 3

Gpr65 morphants have significantly increased levels of runx1 mRNA detected by ISH. (A) Representative images of ISH for runx1 in 29 hpf wild-type (blue) and gpr65 MO-injected (orange) embryos, showing the expression in the dorsal aorta. (B) Pixel intensity values of runx1 mRNA in in gpr65 MO-injected embryos (n=16) are significantly higher than in uninjected control siblings (n=16). The coefficients of variation are 34% and 26% for wild-type and morphant groups, respectively. Blue and orange data point correspond to the example images from panel A. The bars represent mean±s.d. *P<0.05 (t-test). The power of the t-test to detect the difference at 0.05 level was 63%.

Expression Data

Gene:	runx1
Fish:	WT WT + MO2-gpr65
Knockdown Reagent:	MO2-gpr65
Anatomical Term:	ventral wall of dorsal aorta
Stage:	Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO2-gpr65
Knockdown Reagent:	MO2-gpr65
Observed In:	ventral wall of dorsal aorta
Stage:	Prim-5

Phenotype Detail

Acknowledgments

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