FIGURE

Fig. 6

ID

ZDB-FIG-180622-32

Publication

Missinato et al., 2018 - Dusp6 attenuates Ras/MAPK signaling to limit zebrafish heart regeneration

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Fig. 6

dusp6 mutant hearts are mildly affected by PDGFR inhibition. (A-D) Hearts at 7?dpa were injected for 6?days with PTKI III (C,D) or vehicle DMSO (A,B) and stained for Mef2c and Pcna to determine cardiomyocyte proliferation. WT hearts (DMSO, n=22; PTKI III, n=19) (A,C) and dusp6^pt30a/pt30a hearts (DMSO, n=21; PTKI III, n=15) (B,D). Arrowheads indicate proliferating cardiomyocytes. (E) Quantification of cardiomyocyte proliferation in WT and dusp6 mutant hearts after injection of PTKI III or DMSO. Each point represents the average proliferation index from one heart. (F-I) AFOG staining of WT or dusp6^pt30a/pt30a hearts at 20?dpa injected for 6?days with PTKI III (WT, n=8; dusp6^pt30a/pt30a, n=12) (H,I) or DMSO (WT, n=10; dusp6^pt30a/pt30a, n=7) (F,G). Dashed line demarcates injury zone. (J) Quantification of fibrotic tissue area in hearts at 20?dpa. (E,J) ****P<0.0001, ***P<0.001, **P<0.01; ns, not significant; one-way ANOVA.

Expression Data

Antibody:	Ab1-mef2
Fish:	WT dusp6^pt30a/pt30a
Conditions:	resection: cardiac ventricle chemical treatment by injection: PDGF receptor tyrosine kinase inhibitor III, resection: cardiac ventricle
Anatomical Term:	cardiac muscle cell
Stage:	Adult

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT dusp6^pt30a/pt30a
Condition:	chemical treatment by injection: PDGF receptor tyrosine kinase inhibitor III, resection: cardiac ventricle
Observed In:	cardiac muscle cell proliferation heart
Stage:	Adult

Phenotype Detail

Acknowledgments

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