Fig. 5

Vegf-Aa knockdown by miR-26a-5p causes proteinuria and edema in afore healthy zebrafish (A): Pictures of tail vessels (a), glomeruli fusing at zebrafish midline (b) and eye vessel plexus (c) of 48 h old zebrafish shows proper vascular development as well as glomerular fusion. Tail and eye vessel pictures were taken of a Tg(l-fabp:DBP-eGFP/Flk-mcherry) transgenic zebrafish with red fluorescent endothelium. Glomerular fusion is shown in a Tg(wt1b:eGFP) transgenic zebrafish that expresses eGFP under control of the wt1b promotor, arrow points at the fused glomerulus). Scale bar?=?100?µM and 300?µM as indicated. (B,C) Quantification of loss of plasma proteins by measuring maximum eye fluorescence of the retinal vessel plexus (B) and quantification of edemous phenotype (C) of Tg(l-fabp:DBP:eGFP) zebrafish larvae at 120 hpf. A control miR mimic (miR-CTRL), a miR-26a-5p mimic (miR-26a-5p) or the combination of miR-26a-5p and Vegf-Aa protein were injected in the cardinal vein at 48 hpf. At this time the tail vasculature, glomerular fusion and retinal vessel formation was already formed; c.v. miR-CTRL n?=?10, c.v. miR-26a-5p n?=?17, miR-26a-5p + Vegf-Aa n?=?13, WT + Vegf-Aa n?=?8, total n?=?48.