FIGURE

Fig. S4

ID

ZDB-FIG-180620-35

Publication

Zhou et al., 2018 - MicroRNA-223 Suppresses the Canonical NF-κB Pathway in Basal Keratinocytes to Dampen Neutrophilic Inflammation

Other Figures

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Fig. S4

Myd88 knock out rescues over-inflammation in miR-223^-/- embryos, related to Figure 3. (A, B) Representative images and quantification of efficiency of transient knock out of GFP using the CRISPR/Cas system. Single guide RNAs targeting GFP were injected into embryos from Tg(lyzC: GFP) together with Cas9 proteins, or Cas9 protein alone. The number of green neutrophils at 3 dpf was counted. (C) CRISPR screening. Two separate sgRNAs were designed for each gene. Pooled sgRNAs targeting one gene or two genes were injected with Cas9 protein into miR-223^-/- embryos. Neutrophil recruitment to the wound at 6 hpw was quantified using gfp sgRNA as a control. Genes in the three pathways were color coded separately.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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