FIGURE

Fig. S5

ID

ZDB-FIG-180608-19

Publication

Lai et al., 2018 - The Hippo pathway effector Wwtr1 regulates cardiac wall maturation in zebrafish

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Fig. S5

The Tead-binding domain of Wwtr1 is required for cardiomyocyte expression of the tp1 Notch reporter. (A) Quantification of nuclei of tp1⁺ cardiomyocytes exhibiting Wwtr1 nuclear localization (Wwtr1⁺) or not (Wwtr1^-). (B) Schematic of constructs to express mKate and Wwtr1, connected by a self-cleavable 2A peptide, under the myl7 promoter. WT wwtr1 was substituted with different variants in the WW domain (WW*: W142A, P145A) and Tead-binding domain (TBD*: S48A; ΔTBD: P46_D65del). (C) Quantification of tp1⁺ and tp1^- wwtr1^-/- hearts after injection with the constructs shown on the x-axis. tp1⁺ mutant hearts show at least one double-labeled cardiomyocyte (i.e. tp1+ and mKate⁺). (D) Representative maximum intensity projections of wwtr1^-/- hearts after injection with the constructs shown in (A). Yellow arrows point to tp1⁺ cardiomyocytes. The out of focus white signal is from endocardial cells at the valve regions (black asterisks). Scale bars, 10 μm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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