ZFIN Image: Lai et al., 2018, Fig. S5

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Figure Caption

Fig. S5

The Tead-binding domain of Wwtr1 is required for cardiomyocyte expression of the tp1 Notch reporter. (A) Quantification of nuclei of tp1⁺ cardiomyocytes exhibiting Wwtr1 nuclear localization (Wwtr1⁺) or not (Wwtr1^-). (B) Schematic of constructs to express mKate and Wwtr1, connected by a self-cleavable 2A peptide, under the myl7 promoter. WT wwtr1 was substituted with different variants in the WW domain (WW*: W142A, P145A) and Tead-binding domain (TBD*: S48A; ΔTBD: P46_D65del). (C) Quantification of tp1⁺ and tp1^- wwtr1^-/- hearts after injection with the constructs shown on the x-axis. tp1⁺ mutant hearts show at least one double-labeled cardiomyocyte (i.e. tp1+ and mKate⁺). (D) Representative maximum intensity projections of wwtr1^-/- hearts after injection with the constructs shown in (A). Yellow arrows point to tp1⁺ cardiomyocytes. The out of focus white signal is from endocardial cells at the valve regions (black asterisks). Scale bars, 10 μm.

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