FIGURE

Fig. 4

ID

ZDB-FIG-180529-52

Publication

Lindsey et al., 2018 - A Whole Brain Staining, Embedding, and Clearing Pipeline for Adult Zebrafish to Visualize Cell Proliferation and Morphology in 3-Dimensions

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Fig. 4

Identifying the proliferative profile across the brain axis following injury using Histogram Analysis. (A) Histogram Analysis workflow using FIJI/IMAGEJ showing a maximum projection derived from horizontal sections and overlayed with a threshold mask to detect only non-black pixels. By segmenting the brain along the A-P axis the mean pixel intensity/segment can be used to represent the mean EdU intensity/segment. (B–E) Mean pixel intensity of EdU per segment plotted as a histogram across the brain axis displaying conspicuous peaks in brain regions where greater EdU labeling (green) is present shown in control (B; n = 5 brains) and 1-dpl (C; n = 4 brains), 3-dpl (D; n = 4 brains), and 7-dpl (E; n = 5 brains) treated animals. The yellow asterisk denotes the lesioned telencephalic hemisphere. Fb, forebrain; Mb, midbrain; Hb, hindbrain. (F,G) EdU intensity plotted across the A-P brain axis compared across all groups (F) and normalized as the percent change from control (G). Scale bars: (B–E) = 500 μm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front. Neurosci.