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ZFIN ID: ZDB-FIG-180508-21
Kell et al., 2018 - Targeted deletion of the zebrafish actin-bundling protein L-plastin (lcp1). PLoS One   13:e0190353 Full text @ PLoS One
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibody:
Fish:
Anatomical Terms:
Stage Range: Long-pec to Day 6
PHENOTYPE:
Fish:
Observed In:
Stage Range: Long-pec to Day 6

Fig. 5

Leukocyte staining is abolished in lcp1 null animals.

A) Flow chart of the experiment. lcp1 incrosses (+/- x +/-) were raised to 6 dpf and then processed for whole-mount immunostaining (head) and genomic DNA isolation (body). B) Representative genotyping results after genotyping PCR and Bsl1restriction enzyme digest. Homozygous wildtype, homozygous null, and heterozygous siblings are easily distinguished. C) Typical fluorescent immunostaining of a superficial leukocyte in wildtype and heterozygous animals. There is intense signal in the entire cytoplasm, including distant cellular projections; in contrast, the nucleoplasm is dark. D) Heterozygous and null siblings of the Charlie line (CH). In heterozygous fish, leukocytes are stained intensely, particularly in the gill area (arrow). In null fish, no leukocytes are visible. A faint, non-specific staining is present in all embryonic skeletal muscle. E) Heterozygous and null siblings of the Foxtrot line (FX). In the null animal, the LCP1-positive cells are undetectable. F) Null animals have neutrophils as seen in 2 dpf caudal fins stained with Sudan Black. G) Null animals have macrophages, as seen in 2 dpf caudal fins from embryos with green macrophages (Tg(mpeg1.1:Dendra2)). Nuclei are counterstained with DAPI.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
Dendra2 lcp1ud1/ud1 ; uwm12Tg standard conditions Long-pec macrophage IFL
lcp1 Ab2-lcp1 lcp1ud1/+ standard conditions Day 6 leukocyte IHC
Ab2-lcp1 lcp1ud1/ud1 standard conditions Day 6 Not Detected leukocyte IHC
Ab2-lcp1 lcp1ud2/+ standard conditions Day 6 leukocyte IHC
Ab2-lcp1 lcp1ud2/ud2 standard conditions Day 6 Not Detected leukocyte IHC
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
lcp1ud1/ud1 standard conditions Long-pec neutrophil present, normal
Day 6 leukocyte lcp1 expression absent, abnormal
lcp1ud1/ud1 ; uwm12Tg standard conditions Long-pec macrophage present, normal
lcp1ud2/ud2 standard conditions Day 6 leukocyte lcp1 expression absent, abnormal
lcp1ud2/ud2 ; uwm12Tg standard conditions Long-pec macrophage present, normal
Acknowledgments:
ZFIN wishes to thank the journal PLoS One for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS One