FIGURE

Fig. 3

ID

ZDB-FIG-180502-14

Publication

Barsh et al., 2017 - Vagus Motor Neuron Topographic Map Determined by Parallel Mechanisms of hox5 Expression and Time of Axon Initiation

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Fig. 3

hox5 Expression Distinguishes Anterior and Posterior mX Neurons

(A) hoxb5a is expressed in the posterior mX territory. RNA in situ hybridization of 28 hpf embryo for hoxb5a expression (purple) followed by immunostaining against Tg(isl1:eGFPCAAX) to label motor neurons (green) is shown. Dotted lines in (A)?(D) indicate anterior limit of hoxb5a expression. See also Figure S2.

(B?E) hoxb5a^GFP is expressed in posterior mX neurons, PA6, and PA7. hoxb5a^GFP (green) and Tg(isl1:mRFP) (magenta) at 32 hpf (B), 2 dpf (C), or 3 dpf (D and E) are shown.

(F and G) Anterior mX neurons marked by lineage dye (cyan) and isl1:mRFP (magenta) transplanted homotopically (F) or heterotopically (G) are hoxb5a^GFP-negative (arrow in F) or hoxb5a^GFP-positive, respectively (arrow, green in G and black in G?). Host motor neurons express Tg(isl1:mRFP) (magenta).

(H) Quantification of (F) and (G). Analysis was done by Fisher?s exact test. Ant ? ant: n = 22 neurons, 6 embryos; ant ? post: n = 28 neurons, 9 embryos; post ? post: n = 13 neurons, 5 embryos.

Expression Data

Genes:	EGFP GFP hoxb5a mRFP
Fish:	fh474Tg hoxb5a^fh468Tg; fh1Tg
Anatomical Terms:	cranial nerve X cranial nerve X axon cranial nerve X posterior region pharyngeal arch 6 pharyngeal arch 7
Stage Range:	Prim-5 to Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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