FIGURE

Fig. 1

ID

ZDB-FIG-180502-12

Publication

Barsh et al., 2017 - Vagus Motor Neuron Topographic Map Determined by Parallel Mechanisms of hox5 Expression and Time of Axon Initiation

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Fig. 1

Topographic Mapping by Vagus Motor Neurons

(A) Lateral view of a zebrafish embryo expressing Tg(isl1:Kaede) at 3 dpf. Brackets, cranial motor nuclei; ov, otic vesicle; PA, pharyngeal arches. Asterisk marks a subset of vagus sensory cells labeled by islet1.

(B) mX axon branches innervate PAs sequentially. See Movie S1. Gray arrowhead indicates the glossopharyngeal motor nerve (mIX).

(C) tcf21:mCherry⁺ PA muscle precursors (magenta) appear sequentially prior to mX axon entry. White bracket indicates mX nucleus expressing isl1:eGFPCAAX (green). See Movie S2.

(D–H) mX neurons are arranged in a topographic map. Single neuron labeled by Tg(isl1:eGFPCAAX) in magenta or black (D′–H′) on Tg(isl1:mRFP) background (green). Dotted lines indicate the length of the mX territory. (D) Neuron in region 1 projecting to PA4; (E) neuron in region 2 projecting to PA5; (F) neuron in region 2 projecting to PA6; (G) neuron in region 3 projecting to PA7; (H) neuron in region 6 projecting to the visceral branch.

(I) Quantification of (D)–(H). mX territory was divided into 10 equal-length bins along the A-P axis.

In all figures, anterior is left and dorsal is up. The scale bars represent 50 μm. See also Figure S1.

Expression Data

Genes:	EGFP Kaede mCherry
Fish:	ch103Tg fh474Tg; pd108Tg
Anatomical Terms:	cranial nerve V cranial nerve V axon cranial nerve VII cranial nerve VII axon cranial nerve X cranial nerve X axon pharyngeal arch 4 pharyngeal arch 5 pharyngeal arch 6
Stage Range:	Prim-5 to Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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