Fig. 4

New features in the tail fin bone observed by microCT-scan and FIB-SEM. a. Confocal fluorescence microscopy observations of the tail stained by a calcein solution. b) volume rendering and c) cross-section of the volume rendering using CT-scan of the tail fin at 30 dpf, at a resolution of 2.5 μm, shows unknown high density structures (arrowheads). d, e and f are FIB-SEM scan and 3D reconstructions, respectively, of the forming region of a mineralized tail in cross section (d and e) and reconstructed longitudinal section (f) at a resolution of 20 nm. The unidentified high density structures (arrowheads) are close to the lepidotrichia (bone) and between two actinotrichia (collagen bundles) (white asterisks in d).