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Publication: Silvent et al., 2017 - Zebrafish skeleton development: High resolution micro-CT and FIB-SEM block surface serial imaging for phenotype identification
All Figures: Figures for Silvent et al., 2017

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Fig. 4 New features in the tail fin bone observed by microCT-scan and FIB-SEM. a. Confocal fluorescence microscopy observations of the tail stained by a calcein solution. b) volume rendering and c) cross-section of the volume rendering using CT-scan of the tail fin at 30 dpf, at a resolution of 2.5 μm, shows unknown high density structures (arrowheads). d, e and f are FIB-SEM scan and 3D reconstructions, respectively, of the forming region of a mineralized tail in cross section (d and e) and reconstructed longitudinal section (f) at a resolution of 20 nm. The unidentified high density structures (arrowheads) are close to the lepidotrichia (bone) and between two actinotrichia (collagen bundles) (white asterisks in d).

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